homologhmm A hidden Markov Model sequence and alignment decoder (c) 2005 Lukas Kall. The program is distributed under GPL, see http://gnu.org for details The program uses the BioJava (http://biojava.org) which is distributed under LGPL and jPhobius a java implementation of Phobius a hidden Markov Model capable of predicting both Transmembrane Topology and Signal peptides (c) 2004 Lukas Kall, Anders Krogh, Erik Sonnhammer. Please cite: Lukas Käll, Anders Krogh and Erik Sonnhammer. An HMM posterior decoder for sequence feature prediction that includes homology information Bioinformatics, 21 (Suppl 1):i251-i257, June 2005. or Lukas Käll, Anders Krogh and Erik L. L. Sonnhammer. A Combined Transmembrane Topology and Signal Peptide Prediction Method. Journal of Molecular Biology, 338(5):1027-1036, May 2004. PREREQUISITES ============= Unix/Linux Perl 5.6 or later Java 1.5.0 or later To retrieve homologs with the script blastget BioPerl(bioperl.org) is needed. If jPhobius is to be used: The phobius.model file from your licensed copy of phobius INSTALL ======= Change (and create) directory to the directory where you want to install homologhmm (e.g. /usr/local/share/jphobius ), and unpack the tar file with the command: cat /path_to_tarfile/jphobius.tar.gz | tar xz make a link from the file jphobius and homologhmm to a directory included in your standard path. ln -s /usr/local/share/jphobius/jphobius /usr/local/bin/phobius ln -s /usr/local/share/jphobius/homologhmm /usr/local/bin/homologhmm Make sure that java is available in the standard path NOTE: To vitalize jPhobius please copy the file phobius model into the homologhmm directory (/usr/local/share/jphobius)