Phobius — PolyPhobius

Homology-supported prediction (PolyPhobius)

Transmembrane topology is better conserved than sequence, so aligning a protein with its homologues sharpens the prediction.

Paste your protein sequence here in FASTA format:

Or upload a file:

Input format Aligned FASTA — the prediction is reported for the first sequence Single sequence — search Swiss-Prot for homologues and align them automatically

Note: the automatic search uses Swiss-Prot with BLAST. The original server searched UniProt/TrEMBL, so results from this path will not match historical ones. Supplying your own alignment is the reproducible option.

Output format Short — one line per protein Long — feature table, no graphics Long with graphics Graphics only — probabilities in alignment coordinates (aligned FASTA input only)

Up to 100 sequences and 50,000 residues per request. For larger jobs, use the standalone package.