A combined transmembrane topology and signal peptide predictor

Ask Phobius!
Normal prediction Constrained prediction PolyPhobius Instructions Download

Homology supported predictions (PolyPhobius)

Paste your protein sequence here in Fasta format:

Or: Select the sequence file you wish to use

Select input format:

Individual fasta sequences (to be blasted aginst uniprot_trembl)
Aligned fasta (only the first sequence will appear in the final prediction)

Select output format:

Long without Graphics
Long with Graphics
Graphics only (PLP plot with alignment coordinates, only with aligned fasta)