Phobius

A combined transmembrane topology and signal peptide predictor

Normal prediction Constrained prediction PolyPhobius Instructions Download API

Constrained prediction

Fix the location of residues you already know about, and Phobius will find the most probable topology consistent with them.

Paste your protein sequence here in FASTA format (only the first sequence is used):

Or upload a file:

Constraints

Positions separated by spaces. Ranges are written 33-40. Use C for the C-terminus.

Membrane
Cytoplasmic loop
Non-cytoplasmic loop

Constrain the N-terminus to be a signal peptide

Output format Short — one line per protein Long — feature table, no graphics Long with graphics

Up to 100 sequences and 50,000 residues per request. For larger jobs, use the standalone package.

Phobius predicts transmembrane topology and signal peptides from a single hidden Markov model. Please cite Käll, Krogh & Sonnhammer, J Mol Biol 338:1027-1036 (2004).