Phobius

A combined transmembrane topology and signal peptide predictor


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Normal prediction Constrained prediction PolyPhobius Instructions Download

Constrained prediction

Paste your protein sequence here in Fasta format (only the first sequence will be used):


Or: Select the sequence file you wish to use

Enter the positions of the amino acids you want to constrain in a localization below, separated by space. Use C for the C-terminus. It is possible to specify a range of positions i.e. 33-40.

Membrane:
Cytoplasmic loop:
Non-cytoplasmic loop:
Check here if you want to constrain the N-terminus as a signal peptide

Select output format:

Short
Long without Graphics
Long with Graphics